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Ultra Pure Grade Catalog NO. ST A1100
Agarose
Store dry at room temperature
Descriptions:
Size: |
Formulation: |
|
White Powder |
|
White Powder |
Introduction:
Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID).
Data:
EEO(electroendosmosis)(-Mr) |
0.1-0.15 |
Water content |
≤10% |
Sulfate(SO2) |
0.15-0.2% |
Gel Strength(3%gel) |
≥1400/cm2 |
Gel Strength(1.5%gel) |
≥500/cm2 |
Melting point(1.5%gel) |
|
Gelling point(1.5%gel) |
|
Foreign activity
|
DNase, RNase, none detected
|
Analysis Note :
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Preparation of Agarose Gels for DNA separations:
Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer, e.g. for an 0.8% gel, add
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