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生物器材论坛 http://www.bio-equip.com/
简介:
迈晨公司开发生产的“荧光素酶活性检测试剂盒”The
Luciferase Assay Kit provides a sensitive, quick, and quantitative measurement
of the activity of firefly luciferase produced in cultured mammalian cells. The
kit contains a cell lysis buffer, an assay buffer, and the luciferase substrate.
The cell lysis buffer allows efficient extraction of luciferase from many types
of cultured cells within 5 minutes. The luciferase assay is performed by adding
the luciferase substrate to the cell lysate premixed with the assay buffer.
Luciferase in the cell lysate catalyzes the chemiluminescent reaction, which
emits light that is readily measurable with a luminometer.