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标题:二喹啉甲酸(BCA)蛋白检测试剂盒

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O-R-T 发表于:2014-7-21 10:49:00

Description: BCA serves the purpose of the Folin reagent in the Lowry assay, namely to react with complexes between copper ions and peptide bonds to produce a purple end product. The advantage of BCA is that the reagent is fairly stable under alkaline conditions, and can be included in the copper solution to allow a one step procedure. A molybdenum/tungsten blue product is produced as with the Lowry. In addition to standard liquid handling supplies a visible light spectrophotometer is needed with transmission set to 562 nm. Glass or polystyrene (cheap) cuvettes may be used. The BCA is used for the same reasons the Lowry is used. Stoscheck (1990) has suggested that the BCA assay will replace the Lowry because it requires a single step, and the color reagent is stable under alkaline conditions.

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