标题：[原创]支原体检测套装
Description:
PCR-based detection of mycoplasma contamination of cell
culture.
Mycoplasma is a common and serious contaminant of cell
cultures. It has been shown that more than 30% of cell cultures in the
laboratory are infected with Mycoplasma. In continuous cell cultures,
contaminating Mycoplasma may grow slowly without killing the cells but
affecting various parameters including altered cellular proliferation and
viability, morphological changes, cell transformation, mimicking virus
infection, and inresponsiveness to drug treatment, etc., and ultimately leading
to unreliable results. Mycoplasma detection is an important and necessary
quality control measure.
Many of the testing procedures have been developed, which
include DNA staining, enzyme-linked immunosorbent assay (ELISA), polymerase
chain reaction (PCR), and PCR-based ELISA. M&C Gene Technology provide our research
community with reliable reagents and simple protocol, which allow for rapid and
highly reproducible detection of mycoplasma contamination.
The primers used in this kit anneal to conserved regions
of the Mycoplasma genome, allowing detection of the most common species of
Mycoplasma (including M. opalescens, M. arginini; M. fermentans; M. caviae, M.
hyorhinis, M. indiense, M. orale, Acholeplasma laidlawii and many more – see
table 2 below).