标题：血管内皮生长因子 价格实惠 品质有保证

VascularEndothelial Growth Factor (121 a.a.),Basic

Catalogue Number: CC108

Description: Vascular endothelial growth factor is an
important signaling protein involved in both vasculogenesis and angiogenesis.
As its name implies, VEGF activity has been mostly studied on cells of the
vascular endothelium, although it does have effects on a number of other cell
types (e.g. stimulation monocyte/ macrophagemigration, neurons, cancer cells,
kidney epithelial cells).VEGF mediates increased vascular permeability, induces
angiogenesis, vasculogenesis and endothelial cell growth, promotes cell
migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate
endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and
increases microvascular permeability and was originally referred to as vascular
permeability factor. Elevated levels of this protein are linked to POEMS
syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been
associated with proliferative and nonproliferative diabetic retinopathy.
Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is
a double, non-glycosylated, polypeptide chain containing 121 amino acids and
having a molecular mass of 28423 Dalton.
VEGF121 circulates more freely than other VEGF forms, which bind more tightly
with vascular heparin sulfates. The VEGF-121 is purified by proprietary
chromatographic techniques.

Source: Escherichia Coli.

Physical Appearance: Sterile Filtered Clear
Solution.

Formulation: 10ug/ml solution in 10mM sodium phosphate buffer (pH=8.0) with 0.1% BSA
added as stabilizer.

Stability: bFGF should be stored at 4°C between 2-7 days and for future use below -20°C. Please avoid freeze-thaw cycles.

Suitability: Cell culture tested

Purity: Greater than 98.0% as determined by: (a)
Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Biological Activity: Determined by the
dose-dependent stimulation of the proliferation of human umbilical vein endothelial
cells (HUVEC) using a concentration range of 0.2-0.4 ng/ml.

Amino acid sequence:

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENCDKPRR