标题：迈晨科技销售成纤维细胞生长因子（碱性），细胞生长添加剂

Fibroblast Growth Factor (basic), Human Recombinant

Cell
Culture Reagents-Growth Supplements
 

Catalogue Number: CC107

Description: FGF-basic is a member of the fibroblast growth
factor (FGF) family. FGF family members possess broad mitogenic and cell survival
activities, and are involved in a variety of biological processes, including
embryonic development, cell growth, morphogenesis, tissue repair, tumor growth
and invasion. This protein functions as a modifier of endothelial cell
migration and proliferation, as well as an angiogenic factor. It acts as a
mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro,
thus is thought to be involved in organogenesis. Three alternatively spliced
variants encoding different isoforms have been described. The heparin-binding
growth factors are angiogenic agents in vivo and are potent mitogens for a
variety of cell types in vitro. There are differences in the tissue
distribution and concentration of these 2 growth factors. Fibroblast Growth
Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single,
non-glycosylated, polypeptide chain containing 147 amino acids and having a
molecular mass of 16539 Dalton.
The FGF2 is purified by proprietary chromatographic techniques.

Source: Escherichia Coli.

Physical Appearance: Sterile Filtered Clear
Solution.

Formulation: 100g/ml solution in 10mM sodium phosphate buffer (pH=8.0) with 0.1% BSA
added as stabilizer.

Stability: bFGF should be stored at 4°C between 2-7 days and for future use below -20°C. Please avoid freeze-thaw cycles.

Suitability: Cell culture tested

Purity: Greater than 95.0% as determined by: (a)
Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Biological Activity: The ED50, measured in a
mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be less then
0.1ng/ml, corresponding to a specific activity of 3 x 10⁶units/mg.

Amino acid sequence:

MPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS