标题：M&C有供应ECGS，有需求的来关注下

Endothelial
Cell Growth Supplement (ECGS)

Cell
Culture-Growth Supplements

Catalogue Number: CC019

Description: Over
the last three decades cell biologists have made tremendous gains on culturing
non-fibroblast cell types. The finding that culture media containing serum were
in fact growth inhibitory to many cell types, especially those of epithelial
origin, necessitated the use of alternative supplementation. Although we are
now able to use many purified growth factors and hormones, most primary culture
systems still require some additional supplementation from crude tissue
extracts for extended passaging especially at monolayer densities. ECGS
continues to be the most successful of the tissue-sourced extracts to date and
has allowed researchers to study normal primary cell types, particularly endothelial
cell of a variety of origins. Because this EGCS prep is such a highly
concentrated source of putative mitogens and growth factors it can be used at
10-20 times lower the volumes required of serum providing savings in costs. MACGENE’s
ECGS is made from fresh, unfrozen glands to ensure maximal mitogenic response.
We do not lyophilize the extract since lyophilization has been shown to reduce
the spectrum of biological activity. Our product is an aqueous extract
formulated at 14 mg/ml active protein. Growth tests on vascular endothelial
cells of mammalian origin indicate an optimal concentration range of 35 ug/ml for clonal growth and 70 ug/ml or 5ml per liter medium, for dense monolayers.
Please note that proper hormone supplementation is required to
ensure the best results. Please note that because this is a complex mixture of
factors, different cell types may require unique levels especially when using
alternative basal media and supplements.

Formulation: Sterile filtered,
water soluble fraction

Protein Content: 14 mg/mL

Endotoxin: <20 EU/mL

Pack Size: 1 mL

Storage/Stability: -20 oC for up to 2 years. Repeated freezing and thawing is not
recommended.