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生物器材论坛 http://www.bio-equip.com/
在正常细胞中，磷脂酰丝氨酸（PS）只分布在细胞膜脂质双层的内侧，而在细胞凋亡早期，细胞膜中的磷脂酰丝氨酸（PS）由脂膜内侧翻向外侧。Annexin V是一种分子量为35~36kD的Ca2+依赖性磷脂结合蛋白，与PS有高度亲和力，故可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。因此Annexin V被作为检测细胞早期凋亡的灵敏指标之一。将Annexin V进行荧光素FITC或PE标记，或者用生物素biotin标记，以标记了的Annexin V作为探针，利用荧光显微镜或流式细胞仪可检测细胞凋亡的发生。
PI（碘化丙锭）是一种核酸染料，它不能透过完整的细胞膜，但在凋亡中晚期的细胞和死细胞，PI能透过细胞膜而使细胞核染红。因此将ANNEXIN V与PI配用，就可以将凋亡中的早晚期细胞（ANNEXIN+/PI-)与死细胞(ANNEXIN+/PI+)区分开来。
订购信息如下：
ALX-209-002-T020 | Annexin V (human) (recombinant) (Biotin) |
ALX-209-250-T020 | Annexin V (human) (recombinant) (FITC) |
ALX-209-251-T100 | Annexin V (human) (recombinant) (R-PE) |
ALX-209-252-T020 | Annexin V (human) (recombinant) (APC) |
ALX-209-256-3100 | Annexin V (human) (recombinant) (FITC) | |
ALX-209-257-T100 | Annexin V (mouse) (recombinant) (Alexa Fluor® 488) | |
ALX-209-258-T100 | Annexin V (human) (recombinant) (ATTO 488) | |
ALX-209-259-T100 | Annexin V (human) (recombinant) (ATTO 647N) | |
ALX-804-100B-T100 | Annexin V (human), mAb (VAA-33) (Biotin) | |
ALX-804-100-C100 | Annexin V (human), mAb (VAA-33) (Purified) |
ALX-804 | Annexin V (human), mAb (VAA-33) (FITC) |
BV-K129-400 | Annexin V-PE-Cy5 Apoptosis Detection Kit |
ALX-850-020-KI01 | Annexin V-FITC Apoptosis Detection Kit |
ALX-850-031-KI02 | Annexin V-Biotin Apoptosis Detection Kit |
ALX-850-049-KI01 | Annexin V (human) ELISA Kit |
ALX-850-250-KI01 | Annexin V-FITC Apoptosis Detection Kit |
ALX-850-251-KI03 | Annexin V-Biotin Apoptosis Detection Kit |
ALX-850-252-KI01 | Annexin V-Cy3 Apoptosis Detection Kit |
ALX-850-253-KI03 | Annexin V-EGFP Apoptosis Detection Kit |
ALX-850-254-KI01 | Annexin V-Cy5 Apoptosis Detection Kit |
ALX-850-255-KI02 | Annexin V-FITC Apoptosis Detection Kit Plus |
ALX-850-256-KI01 | Annexin V-PE Apoptosis Detection Kit |
ALX-850-257-KI02 | Annexin V-Cy3 Apoptosis Detection Kit Plus |
ALX-850-232-KI01 | MitoCapture™ Mitochondrial Apoptosis Detection Kit |
ALX-850-244-KI01 | CaspSCREEN™ Flow Cytometric Apoptosis Detection Kit |