共1 条记录, 每页显示 10 条, 页签: [1]

标题：[原创]美国SignaGen预优化转染试剂—C6

1楼

mzqsw 发表于：2011-2-26 22:12:00

此主题相关图片如下：预优化.jpg

Description

GenJet™ DNA In Vitro Tranfection Reagent for C6 Glioma cell is pre-optimized for transfecting C6 glioma cells. The C6 glioma cell line is a widely used cell line in neurobiological research. Compared to primary neural cells, the C6 cell line offers some major advantages like easy accessibility and culture as well as a good availability of high cell numbers. Nevertheless, the biggest disadvantage of the C6 cells is that they are difficult-to-transfect with commonly used non-viral transfection methods. The pre-optimized transfection reagent simplifies the transfection of C6 cells. Transfection efficiencies of over 60% can be easily achieved with excellent cell viabilities of above 90%.

Refer to the following optimal transfection conditions for maximal transfection efficiency on C6 glioma cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)

~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours

EGFP
pEGFP-N3 (CMV promoter)
60%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet

中国总经销：济南美中清水湾生物科技有限公司

E-mail: jnqsw@163.com

电话：0531-85912162

共1 条记录, 每页显示 10 条, 页签: [1]