标题：[推荐]美国SignaGen转染试剂——LipoD293
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Description
By utilizing our innovative and proprietary lipid conjugation technology, LipoD293™ (Ver. II) is specially designed and formulated by adding proprietary enhancers for transfecting HEK293 cells and other mammalian cells. As a 2nd generation liposome based DNA transfection reagent, LipoD293™ (Ver. II) offers extremely high transfection efficiencies for HEK293
related cells
as well as many mammalian cells with less cytotoxicity. LipoD293™ reagent (Ver. II), upgraded from its previous version with refined chemistry, is 3~4 times more efficient in gene delivery. LipoD293™ reagent (Ver. II), 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Testimonials
I am absolutely thrilled with the LipoD293 transfection reagent! I compared LipoD293 to Lipofectamine for transfection of plasmids to generate viral pseudoparticles and WOW! I recovered 4 times more virus using the LipoD293 reagent than Lipofectamine!! This is wonderful news for me and my mentor as it means that I don't have to spend so much time (and resources) on transfecting 293T cells to recover virus for in vitro experiments. My mentor was also very happy that LipoD293 costs much less than Lipofectamine!! We have already ordered 5 mls of LipoD293 and I have now convinced my lab mates to switch to LipoD293 since I got such good results with it. Thanks for making such a great product!
-------- Christy Lavine, Ph.D., Harvard University
I wanted to update you on the free sample of LipoD293 that you sent to us. I recently transfected some Plat-E's side-by-side with Lipofectamine 2000, and your product out-performed it!!! We are also validating it with HeLa cells, but otherwise we are VERY happy with what we have seen thus far! Thank you for sending us the free sample, it definately made the SALE for us!
--------Catherine Gallo, Ph.D., University of Cincinnati
we have now tried the LipoD293 DNA In Vitro transfection reagent on 293FT cells. In the first flask we used the protocol by Invitrogen provided in the
Virapower box insert (Virapower, pBABE-GFP plasmid, plus Lipofectamine).
In the second flask we used LipoD293 (30 ul/6ml media) with the same amounts of Virapower and plasmid as in the first flask.
The results were stunning:
LipoD293 gave twice as many transformants as Lipofectamine 2000...
-------- A Beta Tester from Wayne State University
I am absolutely thrilled with the LipoD293 transfection reagent! I compared LipoD293 to Lipofectamine for transfection of plasmids to generate viral pseudoparticles and WOW! I recovered 4 times more virus using the LipoD293 reagent than Lipofectamine!! This is wonderful news for me and my mentor as it means that I don't have to spend so much time (and resources) on transfecting 293T cells to recover virus for in vitro experiments. My mentor was also very happy that LipoD293 costs much less than Lipofectamine!! We have already ordered 5 mls of LipoD293 and I have now convinced my lab mates to switch to LipoD293 since I got such good results with it. Thanks for making such a great product!
-------- Christy Lavine, Ph.D., Harvard University
I wanted to update you on the free sample of LipoD293 that you sent to us. I recently transfected some Plat-E's side-by-side with Lipofectamine 2000, and your product out-performed it!!! We are also validating it with HeLa cells, but otherwise we are VERY happy with what we have seen thus far! Thank you for sending us the free sample, it definately made the SALE for us!
--------Catherine Gallo, Ph.D., University of Cincinnati
we have now tried the LipoD293 DNA In Vitro transfection reagent on 293FT cells. In the first flask we used the protocol by Invitrogen provided in the
Virapower box insert (Virapower, pBABE-GFP plasmid, plus Lipofectamine).
In the second flask we used LipoD293 (30 ul/6ml media) with the same amounts of Virapower and plasmid as in the first flask.
The results were stunning:
LipoD293 gave twice as many transformants as Lipofectamine 2000...
-------- A Beta Tester from Wayne State University
I am absolutely thrilled with the LipoD293 transfection reagent! I compared LipoD293 to Lipofectamine for transfection of plasmids to generate viral pseudoparticles and WOW! I recovered 4 times more virus using the LipoD293 reagent than Lipofectamine!! This is wonderful news for me and my mentor as it means that I don't have to spend so much time (and resources) on transfecting 293T cells to recover virus for in vitro experiments. My mentor was also very happy that LipoD293 costs much less than Lipofectamine!! We have already ordered 5 mls of LipoD293 and I have now convinced my lab mates to switch to LipoD293 since I got such good results with it. Thanks for making such a great product!
-------- Christy Lavine, Ph.D., Harvard University
I wanted to update you on the free sample of LipoD293 that you sent to us. I recently transfected some Plat-E's side-by-side with Lipofectamine 2000, and your product out-performed it!!! We are also validating it with HeLa cells, but otherwise we are VERY happy with what we have seen thus far! Thank you for sending us the free sample, it definately made the SALE for us!
--------Catherine Gallo, Ph.D., University of Cincinnati
we have now tried the LipoD293 DNA In Vitro transfection reagent on 293FT cells. In the first flask we used the protocol by Invitrogen provided in the
Virapower box insert (Virapower, pBABE-GFP plasmid, plus Lipofectamine).
In the second flask we used LipoD293 (30 ul/6ml media) with the same amounts of Virapower and plasmid as in the first flask.
The results were stunning:
LipoD293 gave twice as many transformants as Lipofectamine 2000...
-------- A Beta Tester from Wayne State University
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