标题：美国SignaGen转染试剂——Polyjet™
Description
Based on our proprietary polymer synthesis technology, PolyJet™ DNA In Vitro Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. PolyJet™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis (Figure 1), leading to much less cytotoxicity. PolyJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.
Figure 1. A Cartoon Showing Biodegradation of PolyJet™ DNA Transfection Reagent After Endocytosis of Transfection Complex
Testimonials: -----Tiffany Wallace, Ph.D., June 9, 2009, NCI / NIH ------Feng Qiao, Ph.D., Jun 12, 2009, NEI / NIH -----Stephanie Murphy, Ph.D., June 29, 2009, Dartmouth College -------Yumi Uetake, Ph.D., August11, 2009, UMASS -------Ge Zhou, Ph.D., August 12, 2009, NYU -------Emily Mcallister, September 3rd, 2009, PBRC
PolyJet Transfection Reagent worked equally as well as lipofectamine 2000, with little evidence of cell death on 293, PC-3 and 22RV1 cells. I will defiantly consider switching over.
I only did side by side with the testing sample (PolyJet) and Lipo2000 with GFP transfection on COS-7 cells. The result was very good. PolyJet was even better than L2K.
I tested the sample of PolyJet on my NIH-3T3 mouse fibroblasts this weekend. The results were much better than Lipofecatmine LTX. I'm attaching a powerpoint slide with my results (I did not quantify the % transfection efficiency, but the pictures get the point across). I found that the protocol for difficult-to-transfect cell lines worked better than the standard protocol.
I had chance to try your product finally. It was great success. I used HeLa cells and got 10% transfection efficiency (<0.1% for Lipofectamine). Thank you! I was wondering if I also try GenJet™ Plus DNA In Vitro Transfection Reagent? According to your website, the reagent works better than regular PolyJet.
I tested PolyJet and it looks great on MDCK. We placed order. Thank you!
We are happy to provide feedback. PolyJet worked very well for us in HepG2 cells, we got approximately 80% efficiency with pMAX GFP plasmid, by following the conditions in your suggested protocol. We ran a comparison with Lipofectamine, which only showed approximately 20-30% transfection efficiency. We are planning experiments and will be ordering more soon.
E-mail: jnqsw@163.com 免费试用
-----Tiffany Wallace, Ph.D., June 9, 2009, NCI / NIH
I only did side by side with the testing sample (PolyJet) and Lipo2000 with GFP transfection on COS-7 cells. The result was very good. PolyJet was even better than L2K.
------Feng Qiao, Ph.D., Jun 12, 2009, NEI / NIH
I tested the sample of PolyJet on my NIH-3T3 mouse fibroblasts this weekend. The results were much better than Lipofecatmine LTX. I'm attaching a powerpoint slide with my results (I did not quantify the % transfection efficiency, but the pictures get the point across). I found that the protocol for difficult-to-transfect cell lines worked better than the standard protocol.
-----Stephanie Murphy, Ph.D., June 29, 2009, Dartmouth College
I had chance to try your product finally. It was great success. I used HeLa cells and got 10% transfection efficiency (<0.1% for Lipofectamine). Thank you! I was wondering if I also try GenJet™ Plus DNA In Vitro Transfection Reagent? According to your website, the reagent works better than regular PolyJet.
-------Yumi Uetake, Ph.D., August11, 2009, UMASS
I tested PolyJet and it looks great on MDCK. We placed order. Thank you!
-------Ge Zhou, Ph.D., August 12, 2009, NYU
We are happy to provide feedback. PolyJet worked very well for us in HepG2 cells, we got approximately 80% efficiency with pMAX GFP plasmid, by following the conditions in your suggested protocol. We ran a comparison with Lipofectamine, which only showed approximately 20-30% transfection efficiency. We are planning experiments and will be ordering more soon.
-------Emily Mcallister, September 3rd, 2009, PBRC
E-mail: jnqsw@163.com 免费试用
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