标题：美国SignaGen转染试剂--LipoD293™ （3）
HEK293 cells transfected with pEGFP-C1 plasmid using LipoD293™ In Vitro DNA Transfection Reagent (Ver. II) (upper panel) and the most popular brand product 293fectin™ of Invitrogen (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection
A comparison of LipoD293™ (Ver. II) and Lipofecatmine LTX (LTX) on generation of Lentivirus (LV). Three cDNAs were co-transfected with LipoD293™ (Ver. II) and Lipofectamine LTX (LTX) into 293FT cells. A GFP vector, pHR-SIN-cppt-CMVEWP, was used to determine titer of LV. 1x105 293F cells per well was plated in to a 24 well plate followed by addition of different of amounts of the vector supernatant, 1 microliter (upper panel) and 10 microliters (lower panel) respectively. 5 days later, the cells was passed with FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of LV generated with LipoD293™ and L2K were quantified to be 8x106 and 3x106 tu/ml respectively
Technical Information & Datasheet
- LipoD293™ (Ver. II) Reagent General Protocol & Data Sheet
- LipoD293™ (Ver. II) Reagent for Lentivirus Production
Testimonials
I am absolutely thrilled with the LipoD293 transfection reagent! I compared LipoD293 to Lipofectamine for transfection of plasmids to generate viral pseudoparticles and WOW! I recovered 4 times more virus using the LipoD293 reagent than Lipofectamine!! This is wonderful news for me and my mentor as it means that I don't have to spend so much time (and resources) on transfecting 293T cells to recover virus for in vitro experiments. My mentor was also very happy that LipoD293 costs much less than Lipofectamine!! We have already ordered 5 mls of LipoD293 and I have now convinced my lab mates to switch to LipoD293 since I got such good results with it. Thanks for making such a great product!
-------- Christy Lavine, Ph.D., Harvard University
I wanted to update you on the free sample of LipoD293 that you sent to us. I recently transfected some Plat-E's side-by-side with Lipofectamine 2000, and your product out-performed it!!! We are also validating it with HeLa cells, but otherwise we are VERY happy with what we have seen thus far! Thank you for sending us the free sample, it definately made the SALE for us!
--------Catherine Gallo, Ph.D., University of Cincinnati
we have now tried the LipoD293 DNA In Vitro transfection reagent on 293FT cells. In the first flask we used the protocol by Invitrogen provided in the
Virapower box insert (Virapower, pBABE-GFP plasmid, plus Lipofectamine).
In the second flask we used LipoD293 (30 ul/6ml media) with the same amounts of Virapower and plasmid as in the first flask.
The results were stunning:
LipoD293 gave twice as many transformants as Lipofectamine 2000...
-------- A Beta Tester from Wayne State University
I am absolutely thrilled with the LipoD293 transfection reagent! I compared LipoD293 to Lipofectamine for transfection of plasmids to generate viral pseudoparticles and WOW! I recovered 4 times more virus using the LipoD293 reagent than Lipofectamine!! This is wonderful news for me and my mentor as it means that I don't have to spend so much time (and resources) on transfecting 293T cells to recover virus for in vitro experiments. My mentor was also very happy that LipoD293 costs much less than Lipofectamine!! We have already ordered 5 mls of LipoD293 and I have now convinced my lab mates to switch to LipoD293 since I got such good results with it. Thanks for making such a great product!
-------- Christy Lavine, Ph.D., Harvard University
I wanted to update you on the free sample of LipoD293 that you sent to us. I recently transfected some Plat-E's side-by-side with Lipofectamine 2000, and your product out-performed it!!! We are also validating it with HeLa cells, but otherwise we are VERY happy with what we have seen thus far! Thank you for sending us the free sample, it definately made the SALE for us!
--------Catherine Gallo, Ph.D., University of Cincinnati
we have now tried the LipoD293 DNA In Vitro transfection reagent on 293FT cells. In the first flask we used the protocol by Invitrogen provided in the
Virapower box insert (Virapower, pBABE-GFP plasmid, plus Lipofectamine).
In the second flask we used LipoD293 (30 ul/6ml media) with the same amounts of Virapower and plasmid as in the first flask.
The results were stunning:
LipoD293 gave twice as many transformants as Lipofectamine 2000...
-------- A Beta Tester from Wayne State University
