标题：[推荐]ANTI-FLAG

Descriptions

Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.

Immunogen

Synthetic FLAG sequence containing peptide DYKDDDDK-GC conjugated through a terminal cysteine thiol to maleimide-activated KLH

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP

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