粪便DNA提取试剂盒 FastDNA Spin Kit For Feces
货号:116570200 品牌 MP Biomedicals
产品详细描述
从人类和动物的粪便样品中有效分离基因组DNA,直接用于PCR
1.多达500mg的粪便样品
2.内含配合FastPrep仪 器使用的理解介质E
3.通常可以从500mg的粪便样品中分离得到10-20μg总DNA
4.去除对下游实验有影响的
快速高效提取粪便DNA,可裂解任何粪便样品。可直接用于PCR等其下游实验,The
FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments,
host cells as well as bacteria, fungi, viruses, protists and other cells
present in fecal samples are easily lysed within 40 seconds. These benchtop
devices use a unique, optimized motion that provide an extremely quick,
efficient and highly reproducible homogenization that surpasses traditional
extraction methods.
Samples are placed into 2.0 mL tubes
containing Lysing Matrix E, a mixture of ceramic and silica particles designed
to efficiently lyse all stool organisms. Stool samples are pre-treated by
washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT
Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were
carefully developed to protect and solubilize nucleic acids upon cell lysis,
facilitate extraction of genomic DNA , and minimize RNA, humic acid or
polyphenol contamination. Following lysis, samples are centrifuged to pellet
debris and lysing matrix. DNA is purified from the supernatant with a
silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for
digestion, electrophoresis, PCR and any other desired application.
产品应用,如下:
The Fast DNA® SPIN Kit for Feces
quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen
human and animal stool samples.
产品属性,如下:
Product Type |
Kits & Assays |
Components |
Lysing Matrix E 50x
2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100
mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash
Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes
50 each, Catch Tubes 50 each |
Instrument
Compatibility |
FastPrep-24; FastPrep
120; FastPrep-96; SuperFastPrep-1 |
Sample Type |
Soil; Feces;
Environmental Water; Wastewater; Sludge |
Sample Volume |
up to 500 mg per prep |
Tube Compatibility |
2 mL |
Matrix Type |
E |
Molecule Enriched |
DNA |
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