高速激光扫描共焦显微镜

LSM 5 LIVE DuoScan

Visions get moving – now with even greater sensitivity, and with photomanipulation in any regions of the image. The LSM 5 LIVE gives scientists working in life sciences a confocal system that helps them to better understand processes in living cells, and to gain a deeper insight into cellular interaction mechanisms. Specifications System Overview We make it visible. Models Upright: Axio Imager Z.1, Axioskop 2 FS MOT; Inverted: Axiovert 200 M RP (Rear Port) or SP (Side Port) Z drive DC motor with optoelectronic coding, smallest increment 25 nm; fast fine focusing solutions as accessories (stage or objective focus with piezo drive); total range approx. 250 µm XY stage (option) Motorized XY scanning stage, with Mark & Find (XYZ) and Tile Scan (mosaic scan) functions, smallest increment 1 µm Accessories Digital microscope camera AxioCam, integration of incubation chambers, micromanipulators, etc. Models One or two confocal detection channels Scanner One galvanometric scanning mirror for ultra-high-speed scanning, optional second scanning mirror for positioning of zoom region Scanning resolution Up to 1.05 Mpixels, also continuously adjustable for several channels Scanning speed Adjustable, up to 120 frames/s at 512 x 512 pixels, faster modes for smaller formats (505 frames/s at 512 x 100 pixels; 1010 frames/s at 512 x 50 pixels); ultra-high-speed line scan mode with > 60 000 lines/s Scanning zoom 0.5x to 2.0x, optical, free XY offset (depending on configuration) Scanning field 18 mm diagonal field (max.) in intermediate image plane with homogeneous illumination Confocal aperture Individually adjustable for each detection channel Detection Up to two confocal channels for fluorescence, with high-sensitivity detectors; transmitted light brightfield mode possible Data depth Selectable between 8 or 12 bits VIS laser module Polarization-preserving single-mode fiber, acousto-optical intensity control (temperature-stabilized); switching time < 5 µs; all lasers in maintenance-free diode or solid-state technology; diode laser 405 nm/50 mW, alternatively 440 nm/15 mW; diode laser 488 nm/100 mW; diode pumped solid-state laser 532 nm/75 mW, diode laser 635 nm/35 mW LSM 5 LIVE Realtime electronics control of microscope, laser modules, scanning modules and other accessories Computer High-end PC with practical RAM and harddisk capacity or top-end PC with ample RAM and ultra-high-speed RAID 0 harddisk system; ergonomic, high-resolution flat-panel monitors 19" (4:3) or 24" (16:10), numerous accessories; Windows XP operating system for multi-user operation Microscopes Scanning modules Laser modules Electronics modules